The women's 4 × 100 metre freestyle relay event at the 2000 Summer Olympics took place on 16 September at the Sydney International Aquatic Centre in Sydney, Australia.
The U.S. women's team dominated the race from the start to break the six-year-old world record and most importantly, to defend an Olympic title in the event. The foursome of Amy Van Dyken (55.08), Dara Torres (53.51), Courtney Shealy (54.40), and Jenny Thompson (53.62) put together a stellar time of 3:36.61 to capture the relay gold medal, shaving off China's 1994 world record by 1.3 seconds. As the Americans celebrated their triumph in the pool, Thompson picked up her eighth career medal to become the nation's most successful woman in Olympic history.